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Borg, G (1998) Borg s perceived exertion and pain scales Champaign, IL: Human Kinetics Boutcher, S H, Fleischer-Curtian, L A, & Gines, S D (1988) The effects of self-presentation on perceived exertion Journal of Sport and Exercise Psychology, 10, 270 280 Boutcher, S H, & Trenske, M (1990) The effects of sensory deprivation and music on perceived exertion and affect during exercise Journal of Sport and Exercise Psychology, 12, 167 176 Boyd, M, Callaghan, J, & Yin, Z (1991, June) Ego involvement and low competence in sport as a source of competitive trait anxiety Paper presented at the North American Society for the Psychology of Sport and Physical Activity, Asilomar, CA Burkhardt, J, Wilkinson, C, Butts, N K, Kirkendall, D, & Seery, A (1982) Effects of arm / leg training on ratings of perceived exertion.

Medicine and Science in Sports and Exercise, 14, 158 Coote, D, & Tenenbaum, G (1998) Can emotive imagery aid in tolerating exertion efficiently Journal of Sports Medicine and Physical Fitness, 38, 344 354 Covington, M V (1984) The self-worth theory of achievement motivation: Findings and implications Elementary School Journal, 85, 5 20 De Meersman, R E (1988) Personality, effort perception and cardiovascular reactivity Neuropsychobiology, 19, 192 194 Demura, S, & Nagasawa, Y (2003) Relations between perceptual and physiological response during incremental exercise followed by an extended bout of submaximal exercise on a cycle ergometer Perceptual and Motor Skills, 96, 653 663 Duda, J L, & Hall, H (2001) Achievement goal theory in sport: Recent extensions and future directions In R N Singer, H A Hausenblas, & C M Janelle (Eds), Handbook of research on sport psychology (pp 417 443) New York: Wiley Duda, J L.

, & Nicholls, J G (1992) Dimensions of achievement motivation in schoolwork and sport Journal of Educational Psychology, 84, 1 10 Duda, J L, Sedlock, D A, Noble, B, Cohen, B, & Chi, L (1990, April) The inf luence of goal perspective on perceived exertion and af fect ratings during sub-maximal exercise Paper presented at the annual meeting of the American Alliance for Health, Physical Education, Recreation, and Dance, New Orleans, LA Edgar, L, & Smith-Hanrahan, C M (1992) Nonpharmacological pain management In J H Watt-Watson & M I Donovan (Eds), Pain management: Nursing perspective (pp 162 199) Sydney, Australia: Mosby Ekkekakis, P, Hall, E E, & Petruzzello, S J (2004) Practical markers of the transition from aerobic to anaerobic metabolism during exercise: Rationale and a case for affect-based exercise prescription Preventive Medicine, 38, 149 159..

Mesenchymal stem cells have a high proliferative capacity and may be subcultured repeatedly. During the process of cell expansion, MSCs maintained in DMEM-LG supplemented only with FBS remain in an undifferentiated state, as indicated by negative results in in vitro assays for osteogenesis (See Section 7). Bruder et al. [1997] maintained hMSCs through as many as 15 passages. At each passage some cells were continued in serum-supplemented medium, while others were placed into an osteoinductive medium also containing dexamethasone (See Section 7). The conservation of osteogenic potential at each passage was demonstrated by increased alkaline phosphatase activity and the formation of mineralized matrix in cells of the latter group. Careful documentation of cell numbers at each passage revealed that hMSCs could undergo an average of 38 population doublings before reaching senescence (as indicated by arrested cell growth and a broad, attened cell morphology). Subcultured MSCs are similar to primary isolates of the cells in that they remain spindle-shaped broblastoid cells. As indicated above, however, passaged MSCs are distributed evenly over the culture dish rather than being organized into colonies. They are also slightly wider than for primary cultures, a feature that becomes more pronounced with additional subcultivation.

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